@ARTICLE{Portet2009a,
  author = {Stéphanie Portet and Norbert Mücke and Robert Kirmse and Jörg Langowski
	and Michael Beil and Harald Herrmann},
  title = {Vimentin intermediate filament formation: in vitro measurement and
	mathematical modeling of the filament length distribution during
	assembly.},
  journal = {Langmuir},
  year = {2009},
  volume = {25},
  pages = {8817--8823},
  number = {15},
  month = {Aug},
  abstract = {The salt-induced in vitro assembly of cytoplasmic intermediate filament
	(IF) proteins such as vimentin is characterized by a very rapid lateral
	association of soluble tetrameric subunits into 60-nm-long full-width
	"unit-length" filaments (ULFs). We have demonstrated for this prototype
	IF protein that filament elongation occurs by the longitudinal annealing
	of ULFs into short IFs. These IFs further longitudinally anneal and
	thus constitute a progressively elongating filament population that
	over time yields filaments of several microm in length. Previously,
	we provided a mathematical model for the kinetics of the assembly
	process based on the average length distribution of filaments as
	determined by time-lapse electron and atomic force microscopy. Thereby,
	we were able to substantiate the concept that end-to-end-annealing
	of both ULFs and short filaments is obligatory for the formation
	of long IFs (Kirmse, R.; Portet, S.; Mücke, N. Aebi, U.; Herrmann,
	H.; Langowski, J. J. Biol. Chem. 2007, 282, 18563-18572). As the
	next step in understanding the mechanics of IF formation, we have
	expanded our mathematical model to describe the quantitative aspects
	of IF assembly by taking into account geometry constraints as well
	as the diffusion properties of rodlike linear aggregates. Thereby,
	we have developed a robust model for the time-dependent filament
	length distribution of IFs under standard conditions.},
  doi = {10.1021/la900509r},
  institution = {Department of Mathematics, 342 Machray Hall, University of Manitoba,
	Winnipeg, MB, Canada R3L 2N2. portets@cc.umanitoba.ca},
  keywords = {Algorithms; Animals; Biochemistry, methods; Cytoplasm, metabolism;
	Electrons; Intermediate Filaments, metabolism; Ions; Kinetics; Microscopy,
	Atomic Force, methods; Models, Statistical; Models, Theoretical;
	Proteins, chemistry; Time Factors; Vimentin, chemistry},
  language = {eng},
  medline-pst = {ppublish},
  owner = {sportet},
  pmid = {20050052},
url={https://pubs.acs.org/doi/abs/10.1021/la900509r#},
  timestamp = {2013.11.13}
}