by Stéphanie Portet, Ovide Arino, Jany Vassy and Damien Schoëvaërt
Abstract:
The cytoskeleton is a dynamic three-dimensional structure mainly located in the cytoplasm. It is involved in many cell functions such as mechanical signal transduction and maintenance of cell integrity. Among the three cytoskeletal components, intermediate filaments (the cytokeratin in epithelial cells) are the best candidates for this mechanical role. A model of the establishment of the cytokeratin network of an epithelial cell is proposed to study the dependence of its structural organization on extracellular mechanical environment. To implicitly describe the latter and its effects on the intracellular domain, we use mechanically regulated protein synthesis. Our model is a hybrid of a partial differential equation of parabolic type, governing the evolution of the concentration of cytokeratin, and a set of stochastic differential equations describing the dynamics of filaments. Each filament is described by a stochastic differential equation that reflects both the local interactions with the environment and the non-local interactions via the past history of the filament. A three-dimensional simulation model is derived from this mathematical model. This simulation model is then used to obtain examples of cytokeratin network architectures under given mechanical conditions, and to study the influence of several parameters.
Reference:
Organization of the cytokeratin network in an epithelial cell. (Stéphanie Portet, Ovide Arino, Jany Vassy and Damien Schoëvaërt), In J Theor Biol, volume 223, 2003.
Bibtex Entry:
@ARTICLE{Portet2003,
author = {Stéphanie Portet and Ovide Arino and Jany Vassy and Damien Schoëvaërt},
title = {Organization of the cytokeratin network in an epithelial cell.},
journal = {J Theor Biol},
year = {2003},
volume = {223},
pages = {313--333},
number = {3},
month = {Aug},
abstract = {The cytoskeleton is a dynamic three-dimensional structure mainly located
in the cytoplasm. It is involved in many cell functions such as mechanical
signal transduction and maintenance of cell integrity. Among the
three cytoskeletal components, intermediate filaments (the cytokeratin
in epithelial cells) are the best candidates for this mechanical
role. A model of the establishment of the cytokeratin network of
an epithelial cell is proposed to study the dependence of its structural
organization on extracellular mechanical environment. To implicitly
describe the latter and its effects on the intracellular domain,
we use mechanically regulated protein synthesis. Our model is a hybrid
of a partial differential equation of parabolic type, governing the
evolution of the concentration of cytokeratin, and a set of stochastic
differential equations describing the dynamics of filaments. Each
filament is described by a stochastic differential equation that
reflects both the local interactions with the environment and the
non-local interactions via the past history of the filament. A three-dimensional
simulation model is derived from this mathematical model. This simulation
model is then used to obtain examples of cytokeratin network architectures
under given mechanical conditions, and to study the influence of
several parameters.},
institution = {Laboratoire d'Analyse d'Images en Pathologie Cellulaire, Institut
Universitaire d'Hématologie, Hôpital Saint Louis, 1 Avenue Claude
Vellefaux, 75475 Paris, France. sportet@phys.ualberta.ca},
keywords = {Animals; Computer Simulation; Cytoskeleton, physiology; Epithelial
Cells, ultrastructure; Fluorescent Antibody Technique; Intermediate
Filaments, ultrastructure; Keratins, ultrastructure; Microscopy,
Confocal; Models, Organizational},
language = {eng},
medline-pst = {ppublish},
owner = {sportet},
pii = {S0022519303001012},
pmid = {12850452},
timestamp = {2013.11.13}
}